The Recovery of Lethally Irradiated Dogs given Infusions of Autologous Leukocytes Preserved at -80 C.

ETHALLY irradiated dogs usually recover promptly if given several billion fresh, autologous, marrow cells.1’2 Autologous marrow, stored at low temperature in glycerol or dimethyl-sulfoxide, induces a similar recovery.35 Recent studies of lethally irradiated mice have shown that infusions of fresh, isogenic leukocytes, if given in sufficient number, are followed by survival and return of marrow function.#{176}” Atkins, Goodman, Perry and Kerby10 2 have shown that glycerol exerts a protective effect on blood leukocytes stored at low temperatures. This communication reports the survival of dogs given x-irradiation in a dosage of 1200 r and infusions of autologous leukocytes that had been stored at -80 C. in 10 per cent dimethyl-sulfoxide.#{176}